Optimal artificial conditions for keeping and breeding medicinal leeches Hirudo verbana and Hirudo orientalis
DOI:
https://doi.org/10.17420/ap71.549Keywords:
medicinal leeches, optimal environment, breeding, keepingAbstract
The aim of the work is to obtain optimal artificial conditions for the maintenance and breeding of medicinal leeches of the species Hirudo verbana and Hirudo orientalis. 180 medicinal leeches were used in the study: 100 leeches of the H. verbana species and 80 H. orientalis leeches. Four groups of animals were formed: two control and two experimental. Control groups of medicinal leeches were kept using the standard jar method. Experiments according to the developed optimal scheme. As a result of the study, it was found that there were more juveniles obtained from the cocoon per 1 parent leech in the experimental group of medicinal leeches of the H. verbana and H. orientalis species, compared to the control group; appearance of defective cocoons. Mortality of parent leeches during breeding, mortality of juveniles before the first, after the first and sixth feeding decreases on average by more than 50% (P < 0.05) in the experimental group.
References
[1] Abdisa T. 2018. Therapeutic importance of leech and impact of leech in domestic animals. MOJ Drug Design and Development Therapy 2(6): 235‒242. https://doi.org/10.15406/mojddt.2018.02.00068
[2] Amani L., Motamed N., Mirabzadeh A.M., Dehghan S.M., Malek M., Shamsa F., Fatemi E., Amin M. 2021. Semi-solid product of medicinal leech enhances wound healing in rats. Jundishapur Journal of Natural Pharmaceutical Products 16(4): e113910. https://doi.org/10.5812/jjnpp.113910
[3] Aminov R.F., Frolov A.K. 2018. The impact of fetal load of Hirudo verbana saline extract antigens morphometrical, hematological and immunological parameters of rats in the early stages of postembryonic development. Annals of Parasitology 64(1): 13–20. https://doi.org/10.17420/ap6401.127
[4] Aminov R., Frolov A., Aminova, A. 2022. The effect of the biologically complex of a medical leech active substances on the immunosuppressive state of rats. Jordan Journal of Biological Sciences 15(2): 257–261. https://doi.org/10.54319/jjbs/150213
[5] Aminov R.F. 2023. Determination of acute toxicity in rats after exposure to a complex of substances obtained from the medicinal leech (Hirudo verbana Carena, 1820). Biharean Biologist 17 (1): 12–17.
[6] Aminov R.F. 2023. Possible detection of physiological state disorders with the help of medicinal leeches Hirudo verbana Carena, 1820. Annals of Parasitology 69(2): 61–65. https://doi.org/10.17420/ap6902.508
[7] Aminov R.F., Frolov A.K. 2017. Influence of ectoparasite – Hirudo verbana on morphogenetic reactions of the host organism – rattus. Current Trends in Immunology 18: 107–117.
[8] Aminov R. 2023. The influence of the water-salt extract of the medicinal leech Hirudo verbana Carena, 1820 on the general course of embryogenesis in rats after intraperitoneal administration. Studia Biologica 17(2): 85–94. https://doi.org/10.30970/sbi.1702.713
[9] Aminov R., Aminova A. 2021. Indirect effect of substances of the hemophagous parasite Hirudo verbana on the immune system of the host rats. Annals of Parasitology 67(4): 603–610. https://doi.org/10.17420/ap6704.376
[10] Aminov R.F., Frolov O.K.,, Aminova A.S., ,Makyeyeva L.V. 2022. Evaluation of mitotic activity of bone marrow under short-term hypothermia. Problems of Cryobiology and Cryomedicine 32(2): 81–91. https://doi.org/10.15407/cryo32.02.081
[11] Ojo P.О., Babayi H., Olayemi I.K., Peter O.O., Fadipe L.A., Baba E., Izebe K. 2018. Anti-tubercular activities and molecular characterization of salivary extract of leech (Hirudo medicinalis) against Mycobacterium tuberculosis. Journal of Tuberculosis Research 6: 1–9. https://doi.org/10.4236/jtr.2018.61001
[12] Aminov R., Aminova A., Makyeyeva L. 2022. Morphological parameters of spleen and thymus of the male rats on the basis of the hirudological influence of Hirudo verbana. Annals of Parasitology 68(1): 55–60. https://doi.org/10.17420/ap6801.408
[13] Krashenyuk A.I. 2020. Neurotrophic (neural stimulating) and neuromediator effects of Hirudo medicinalis. pathogenetic mechanism of treatment of diseases of the nervous system of the human. Acta Scientific Medical Sciences 4(3): 4–9. https://doi.org/10.31080/ASMS.2020.04.0552
[14] Nowicki A., Jaworska J., Baranski W. 2021. Leech therapy in the treatment of a penile haematoma in a stallion. Veterinární Medicína 66(6): 266–271. https://doi.org/10.17221/163/2020-vetmed
[15] Trenholme H.N., Masseau I., Reinero C.R. 2021. Hirudotherapy (medicinal leeches) for treatment of upper airway obstruction in a dog. Journal of Veterinary Emergency and Critical Care 31(5): 661–667. https://doi.org/10.1111/vec.13094
[16] Yang F., Li Y., Guo S., Pan Y., Yan C., Chen Z. 2021. Hirudo lyophilized powder ameliorates renal injury in diabetic rats by suppressing oxidative stress and inflammation. Evidence-based Complementary and Alternative Medicine: 6657673. https://doi.org/10.1155/2021/6657673
[17] Huang H., Lei R., Li Y., Huang Q., Gao N., Zou W. 2021. Hirudo (leech) for proliferative vitreous retinopathy: a protocol for systemic review and metaanalysis. Medicine 100(3): e24412. https://doi.org/10.1097/md.0000000000024412
[18] Dudhrejiya A.V., Pithadiya S.B., Patel A.B. 2023. Medicinal leech therapy and related case study: overview in current medical field. Journal of Pharmacognosy and Phytochemistry 12(1): 21–31. https://doi.org/10.22271/phyto.2023.v12.i1a.14543
[19] Chhayani K., Daxini P., Patel P. 2023. An overview on medicinal leech therapy. Journal of Pharmacy and Pharmacology 11: 107–113. https://doi.org/10.17265/2328-2150/2023.06.001
[20] Sonani S.R., Dudhamal T.S. 2023. Leech therapy and adjuvant Ayurveda treatment in the management of diabetic foot ulcer with atherosclerosis. BLDE University Journal of Health Sciences 8(1): 192–196. https://doi.org/10.4103/bjhs.bjhs_135_22
[21] Shakouri A., Adljouy N., Abdolalizadeh J. 2018. Anti-cancer activity of liposomal medical leech saliva extract (LSE). In: Proceedings of the 3rd World Congress on Recent Advances in Nanotechnology (RAN’18), April, Budapest, Hungary. Paper No. NDDTE XXX. https://doi.org/10.11159/nddte18.102
[22] Ceylan M., Çetinkaya O., Bulut C. 2021. Acute toxicity of zinc on southern medicinal leech, Hirudo verbana Carena, 1820. Acta Aquatica Turcica 17(3): 421–428. https://doi.org/10.22392/actaquatr.874241
[23] Malek M., Jafarifar F., Aminjan A.R., Salehi H., Parsa H. 2019. Culture of a new medicinal leech: growth, survival and reproduction of Hirudo orientalis Utevsky and Trontelj, 2005 under laboratory conditions. Journal of Natural History 53(11–12): 627–637. https://doi.org/10.1080/00222933.2019.1597200
[24] Ceylan M., Çetinkaya O., Küçükkara R., Akçimen U. 2015. Reproduction efficiency of the medicinal leech Hirudo verbana Carena, 1820. Turkish Journal of Fisheries and Aquatic Sciences 15: 411–418. https://doi.org/10.4194/1303-2712-v15_2_27
[25] Ceylan M., Küçükkara R., Akçimen U. 2019. Effects of broodstock density on reproduction efficiency and survival of southern medicinal leech, Hirudo verbana Carena, 1820. Aquaculture 498: 279–284. https://doi.org/10.1016/j.aquaculture.2018.08.016
[26] Ceylan M., Çetinkaya O., Küçükkara R., Akçimen U. 2015. Reproduction efficiency of the medicinal leech Hirudo verbana Carena,1820. Turkish Journal of Fisheries and Aquatic Sciences 15: 411–418. https://doi.org/10.4194/1303-2712-v15_2_27
[27] Masoumeh M., Fatemeh J., Atabak R.A., Hassan S., Hossein P. 2019. Culture of a new medicinal leech: growth, survival and reproduction of Hirudo orientalis Utevsky and Trontelj, 2005 under laboratory conditions. Journal of Natural History 53(11): 627–637. https://doi.org/10.1080/00222933.2019.1597200
[28] Aminov R.F. 2023. The effect of blood human, domestic and small laboratory animals on the viability and behavior of the medicinal leech. Annals of Parasitology 69(1): 1–6. https://doi.org/10.17420/ap6901.501
[29] Zhang B., Lin Q., Lin J., Chu X., Lu J. 2008. Effects of broodstock density and diet on reproduction and juvenile culture of the leech, Hirudinaria manillensis Lesson, 1842. Aquaculture 276(1–4): 198–204. https://doi.org/10.1016/j.aquaculture.2008.02.003
[30] Manavа М., Ceylan M., Büyükçapar H.M. 2019. Investigation of reproductive efficiency, growth performance and survival of the southern medicinal leech, Hirudo verbana Carena, 1820 fed with mammalian and poultry blood. Animal Reproduction Science 206: 27–37. https://doi.org/10.1016/j.anireprosci.2019.05.004
[31] Ceylan M., Çetinkaya O., Küçükkara, R., Akçimen U. 2015. Reproduction efficiency of the medicinal leech Hirudo verbana Carena, 1820. Turkish Journal of Fisheries and Aquatic Sciences 15: 411–418. https://doi.org/10.4194/1303-2712-v15_2_27
[32] Ceylan M., Küçükkara R., Akçimen U., Yener O. 2017. Reproduction efficiency of the Horse Leech, Haemopis sanguisuga (Linnaeus, 1758). Invertebrate Reproduction and Development 61(3): 182–188. https://doi.org/10.1080/ 07924259.2017.1318096
[33] Ceylan M. 2020. Effects of maternal age on reproductive performance of the southern medicinal leech, Hirudo verbana Carena, 1820. Animal Reproduction Science 218: 106507. https://doi.org/10.1016/j.anireprosci.2020.106507
[34] Bidmal H.R., Sudagar M., Shakouri, M. 2022. The effect of different blood (goat and sheep) on sexual maturity, survival and the production of cocoons and larvae in oriental leech (Hirudo orientalis). Journal of Animal Environment 13(4): 301–306. https://doi.org/10.22034/AEJ.2020.232247.2268
[35] Ceylan M., Küçükkara R., Karataş E. 2023. Effects of cocoon incubation angle on hatching success of medicinal leeches (Hirudo spp.). Invertebrate Reproduction and Development 67(3–4): 121–128. https://doi.org/10.1080/07924259.2023.2241418
[36] Gururaja D., Ballal, A. 2021. Comparative study on traditional and prevailing leech storage methods in Ayurveda leech therapy. Centers Annals Ayurvedic Medicine 10(2): 98–108.
[37] Aminov R., Frolov A., Aminova A. 2021. The influence of the planting time and conditions on the reproductive properties of Hirudo verbana and Hirudo medicinalis. Annals of Parasitology 67(2): 169–174. https://doi.org/10.17420/ap6702.326
[38] Aminov R., Frolov A., Aminova A. 2022. The duration of rest and feeding greatly affects the rebreeding of ectoparasites: Hirudo verbana, Hirudo medicinalis and Hirudo orientalis. Annals of Parasitology 68(4): 721–726. https://doi.org/10.17420/ap6804.479
